Phosphothreonine Antikörper (pThr)

Produktnummer ABIN5596876

Produktdetails anti-Phosphothreonine Antikörper

Target: Phosphothreonine

Bindungsspezifität: pThr

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Phosphothreonine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Kreuzreaktivität (Details): Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphotyrosine or phosphoserine.

Aufreinigung: Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.

Immunogen:

Immunogen: PhosphoThreonine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.

Immunogen Type: Other

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity-purified antibody is suitable for use in ELISA, western blotting and immunoprecipitation assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Western Blot Dilution: 2.0 μg/mL

Immunoprecipitation Dilution: 10.0 μg/mg sample

ELISA Dilution: 0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Phosphothreonine

Target: Phosphothreonine

Abstract: Phosphothreonine Produkte

Substanzklasse: Amino Acid

Hintergrund:

Synonyms: PhosphoThreonine (pT) reactive antibody PhosphoThreonine (pT) polyclonal antibody, PhosphoThreonine antibody produced in rabbit

Background: Phospho-Threonine Antibody detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.