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Esterase Antikörper (HRP)

Reaktivität: Schwein ELISA, WB, IHC Wirt: Kaninchen Polyclonal HRP
Produktnummer ABIN5596851
  • Target Alle Esterase Produkte
    Esterase
    Reaktivität
    Schwein
    Wirt
    • 6
    Kaninchen
    Klonalität
    • 6
    Polyklonal
    Konjugat
    • 3
    • 3
    Dieser Esterase Antikörper ist konjugiert mit HRP
    Applikation
    • 6
    • 6
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    Kreuzreaktivität (Details)
    Cross reactivity against Esterase from other tissues and species may occur but have not been specifically determined.
    Aufreinigung
    Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].
    Immunogen

    Immunogen: Esterase [Porcine Liver]

    Immunogen Type: Native Protein

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Esterase has been assayed against 1.0 μg of Esterase [Porcine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:250 - 1:1,000

    ELISA Dilution: 1:1,000 - 1:5,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Konservierungsmittel
    Gentamicin sulfate
    Vorsichtsmaßnahmen
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    Esterase
    Abstract
    Esterase Produkte
    Synonyme
    Carboxylic ester hydrolase antikoerper, T28C12.4 antikoerper, K11G9.1 antikoerper, K07C11.4 antikoerper, F15A8.6 antikoerper, K11G9.2 antikoerper, K11G9.3 antikoerper, T28C12.5 antikoerper
    Hintergrund

    Synonyms: Liver carboxylesterase, EC=3.1.1.1, Proline-beta-naphthylamidase, Retinyl ester hydrolase

    Background: Esterase is a hydrolase enzyme that splits esters into an acid and an alcohol in a chemical reaction with water called hydrolysis.

    Gene Name: CES3

    Gen-ID
    100626873
    UniProt
    Q29550
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