D Amino Acid Oxidase Antikörper (HRP)

Produktnummer ABIN5596780

Produktdetails anti-D Amino Acid Oxidase Antikörper (HRP)

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reaktivität: Schwein

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser D Amino Acid Oxidase Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against D-Amino Acid Oxidase from other tissues and species may occur but have not been specifically determined.

Aufreinigung: D-Amino Acid Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney].

Immunogen:

Immunogen: D-Amino Acid Oxidase [Pig Kidney]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-D-Amino Acid Oxidase has been assayed against 1.0 μg of D-Amino Acid Oxidase [Pig Kidney] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu D Amino Acid Oxidase

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Andere Bezeichnung: D-Amino Acid Oxidase (DAO Produkte)

Synonyme: DAAO antikoerper, DAMOX antikoerper, OXDA antikoerper, daao antikoerper, oxda antikoerper, damox antikoerper, DAO antikoerper, Dao1 antikoerper, DKFZp469N2232 antikoerper, AI987963 antikoerper, Dao-1 antikoerper, SC5F2A.23c antikoerper, wu:fb55b11 antikoerper, zgc:113922 antikoerper, ABP antikoerper, ABP1 antikoerper, DAO1 antikoerper, KAO antikoerper, MGC114783 antikoerper, Abp antikoerper, Abp1 antikoerper, D-amino acid oxidase antikoerper, D-amino-acid oxidase antikoerper, D-amino-acid oxidase, tandem duplicate 1 antikoerper, amine oxidase, copper containing 1 antikoerper, D-amino-acid oxidase L homeolog antikoerper, DAO antikoerper, Dao antikoerper, dao antikoerper, SCO6740 antikoerper, dao.1 antikoerper, AOC1 antikoerper, PTRG_02224 antikoerper, SJAG_02496 antikoerper, dao.L antikoerper, Aoc1 antikoerper

Hintergrund:

Synonyms: D-amino-acid oxidase DAMOX DAAO DAO EC=1.4.3.3

Background: D-Amino Acid Oxidase enzyme belongs to the FAD dependent oxidoreductase family, and acts on the CH-NH2 group of D-amino acid donors with oxygen as acceptor. It regulates the level of the neuromodulator D-serine in the brain. It has high activity towards D-DOPA and contributes to dopamine synthesis. It could act as a detoxifying agent which removes D-amino acids accumulated during aging. D-Amino Acid Oxidase acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. But does not act on acidic amino acids.

Gene Name: DAO

Gen-ID: 397134

UniProt: P00371