This antibody is expected to recognise Cyclophilin A. This product is expected to recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to PPIA (NP_066953.1).
Kreuzreaktivität (Details)
Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine. Species reactivity (tested):Human.
Aufreinigung
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen
Peptide with sequence from the internal region of the protein sequence according to NP_066953.1. Genename: PPIA
PPIA
Reaktivität: Human, Maus, Ratte
WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-0.3 μg/mL. Approx 18 kDa band observed in Human Lymph Node lysates(calculated MW 18.8 kDa according to NP_066953.1).
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
PPIA
(Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
Cyp1 antikoerper, PPIA-2 antikoerper, fa93g09 antikoerper, ppia antikoerper, wu:fa93g09 antikoerper, wu:fb05e11 antikoerper, wu:fb13h02 antikoerper, wu:fb15a05 antikoerper, CYPA antikoerper, CypA antikoerper, Cypa antikoerper, CYPH antikoerper, CYCA antikoerper, CyP-A antikoerper, MGC75715 antikoerper, 2700098C05 antikoerper, Cphn antikoerper, CyP-18 antikoerper, MT-ND1 antikoerper, MTND1 antikoerper, NADH1 antikoerper, ND1 antikoerper, cypA antikoerper, PPIase A antikoerper, ppial antikoerper, wu:fj18g05 antikoerper, zgc:73102 antikoerper, zgc:86688 antikoerper, peptidylprolyl isomerase A antikoerper, peptidylprolyl isomerase Aa (cyclophilin A) antikoerper, peptidylprolyl isomerase A (cyclophilin A) L homeolog antikoerper, cyclophilin A antikoerper, cyclophilin a antikoerper, peptidylprolyl isomerase A (cyclophilin A) antikoerper, peptidylprolyl isomerase Ab (cyclophilin A) antikoerper, PPIA antikoerper, ppiaa antikoerper, ppia.L antikoerper, Cypa antikoerper, CNB01230 antikoerper, CNB01290 antikoerper, Tc00.1047053506925.300 antikoerper, Tb11.03.0250 antikoerper, CYPA antikoerper, Ppia antikoerper, ppia antikoerper, ppiab antikoerper
Hintergrund
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.Synonyms: CYPA, Cyclophilin-A, Cyclosporin A-binding protein, EC=5.2.1.8, PPIA, PPIase A, Peptidyl-prolyl cis-trans isomerase A, Rotamase A