Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Verwendungszweck
Rabbit polyclonal antibody raised against synthetic peptide of MYL9.
Spezifität
This affinity purified antibody is directed against the regulatory light chain of smooth and non- muscle myosin. This antibody detects both unphosphorylated and monophosphorylated forms of the protein.
Kreuzreaktivität
Human, Maus, Ratte
Produktmerkmale
Antibody Reactive Against Synthetic Peptide.
Immunogen
A synthetic peptide corresponding to amino acids 12-27 of human MYL9.
MYL9
Reaktivität: Human
WB, ELISA, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA (1:5000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Iwasaki, Murata-Hori, Ishitobi, Hosoya: "Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells." in: Cell structure and function, Vol. 26, Issue 6, pp. 677-83, (2002) (PubMed).