Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)
Verwendungszweck
Mouse monoclonal antibody raised against native CD177.
Spezifität
This antibody reacts with CD177 (Neutrophil specific antigen 1), a 60 KDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
Flow Cytometry (20 µL in human blood cells 100 µL in whole blood or 106 cells in a suspension) The optimal working dilution should be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In PBS (0.2 % BSA, 0.09 % sodium azide)
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
Bauer, Abdgawad, Gunnarsson, Segelmark, Tapper, Hellmark: "Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils." in: Journal of leukocyte biology, Vol. 81, Issue 2, pp. 458-64, (2007) (PubMed).
Sachs, Andrei-Selmer, Maniar, Weiss, Paddock, Orlova, Choi, Newman, Preissner, Chavakis, Santoso: "The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31)." in: The Journal of biological chemistry, Vol. 282, Issue 32, pp. 23603-12, (2007) (PubMed).
Mnjoyan, Li, Afshar-Kharghan: "Expression of polycythemia rubra vera-1 decreases the dependency of cells on growth factors for proliferation." in: Haematologica, Vol. 90, Issue 3, pp. 405-6, (2005) (PubMed).