7-Ketocholesterol (7-KC) Antikörper (Biotin)

Produktnummer ABIN5067336

Produktdetails

Target: 7-Ketocholesterol (7-KC)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Spezifität: Specific for 7-ketocholesterol modified proteins.

Aufreinigung: Protein G Purified

Immunogen: Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).

Klon: 3F7

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:50)
• ELISA (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN5067336 was sufficient for detection of 7-Ketocholesterol in 2 μg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Antigendetails

Target: 7-Ketocholesterol (7-KC)

Andere Bezeichnung: 7-Ketocholesterol

Substanzklasse: Hormone

Hintergrund: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 7-ketocholesterol (7-KC) is a biomarker of lipoprotein cholesterol oxidation.