Amyloid beta Precursor Protein Binding Protein 1 (AA 229-534) Antikörper

Produktnummer ABIN5013120

Produktdetails

Target: Amyloid beta Precursor Protein Binding Protein 1

Bindungsspezifität: AA 229-534

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Verwendungszweck: Polyclonal Antibody to Amyloid Beta Precursor Protein Binding Protein 1 (APPBP1)

Spezifität: The antibody is a rabbit polyclonal antibody raised against APPBP1. It has been selected for its ability to recognize APPBP1 in immunohistochemical staining and western blotting.

Kreuzreaktivität: Maus, Schwein, Ratte

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Amyloid Beta Precursor Protein Binding Protein 1 (APPBP1) corresdonding to Thr229~Leu534 with N-terminal His Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western blotting: 0.5-2 μg/mL

1:160-640 Immunohistochemistry: 5-20 μg/mL

1:16-64 Immunocytochemistry: 5-20 μg/mL

1:16-64 Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Referenzen

Naik, Lam, Parija, Prakash, Jiramongkol, Adhya, Parida, Mishra: "NEDDylation negatively regulates ERRβ expression to promote breast cancer tumorigenesis and progression." in: Cell death & disease, Vol. 11, Issue 8, pp. 703, (2020) (PubMed).

Antigendetails

Target: Amyloid beta Precursor Protein Binding Protein 1

Synonyme: A-116A10.1 antikoerper, APPBP1 antikoerper, ula-1 antikoerper, NEDD8 activating enzyme E1 subunit 1 antikoerper, NAE1 antikoerper

Hintergrund: NAE1, HPP1, Ula-1, NEDD8-Activating Enzyme E1 Regulatory Subunit 1, Amyloid protein-binding protein 1, Proto-oncogene protein 1