PLEKHM1
Reaktivität: Human
IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
Cy3
Applikationshinweise
ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS containing 0.02 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody (in aliquots) at -20 °C.
Target
PLEKHM1
(Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 1 (PLEKHM1))
AP162 antikoerper, B2 antikoerper, OPTB6 antikoerper, BC038943 antikoerper, D330036J23Rik antikoerper, pleckstrin homology and RUN domain containing M1 antikoerper, pleckstrin homology domain containing, family M (with RUN domain) member 1 antikoerper, PLEKHM1 antikoerper, Plekhm1 antikoerper
Hintergrund
PLEKHM1 is a member of the M family of Pleckstrin homolog domain-containing proteins, a group of proteins containing a RUN domain, two pleckstrin homology domains, and a cysteine-rich domain. It was identified through segregation analysis as a cause of osteopetrosis in humans. PLEKHM1 co-localizes with Rab7 to late endosomal/lysosomal vesicles in HEK293 and osteoclast-like cells, with this co-localization dependent on the prenylation of Rab7. Monocytes from a patient homozygous for a mutated form of PLEKHM1differentiated into osteoclasts normally, but failed to form ruffled borders and showed little evidence of bone resorbtion when cultured on dentine discs. Another mutation of PLEKHM1 impaired vesicular acidification and increased TRACP secretion in osteoclasts, suggesting that PLEKHM1 has critical roles in endosomal maturation and may be important in osteoclast-osteoblast cross-talk.Synonyms: KIAA0356, PH domain-containing family M member 1, Pleckstrin homology domain-containing family M member 1