HSF1 Antikörper

Produktnummer ABIN498574

Produktdetails anti-HSF1 Antikörper

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of HSF1 protein. (region surrounding Val297)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Andere Bezeichnung: Heat Shock Factor 1 / HSF1 (HSF1 Produkte)

Synonyme: hsf1 antikoerper, Hsf antikoerper, xhsf1 antikoerper, AA960185 antikoerper, HSTF1 antikoerper, HSF1 antikoerper, HSF antikoerper, HSF1c antikoerper, hsf1a antikoerper, hsf1b antikoerper, zHSF1a antikoerper, zHSF1b antikoerper, hstf1 antikoerper, heat shock factor antikoerper, Heat Shock Factor antikoerper, heat shock factor 1 antikoerper, heat shock transcription factor 1 antikoerper, heat shock transcription factor 1 S homeolog antikoerper, hsf1 antikoerper, hsf-1 antikoerper, Hsf1 antikoerper, HSF1 antikoerper, hsf1.S antikoerper

Hintergrund: Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1

Molekulargewicht: approx. 82 kDa

Gen-ID: 3297

NCBI Accession: NP_005517

UniProt: Q00613