KEAP1 Antikörper
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- Target Alle KEAP1 Antikörper anzeigen
- KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser KEAP1 Antikörper ist unkonjugiert
- Applikation
- Bitte anfragen
- Produktmerkmale
- The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintains cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5).The NRF2 repressor KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain (6,7). The KELCH domain is required for interacting with NRF2 and the BTB/POZ domain functions in binding Cul3 E3 ubiquitin ligase (8-10). Under normal conditions, the complex leads to the cytoplasmic sequestration and ubiquitin-mediated proteasomal degradation of NRF2. Electrophilic modification of KEAP1 leads to disassociation of the NRF2/KEAP1 complex. KEAP1 also targets the down regulation of NF-κB activity by targeting IKKβ degradation (11).
- Aufreinigung
- Affinity purification
- Immunogen
- Recombinant protein of human KEAP1.
- Isotyp
- IgG
- Top Product
- Discover our top product KEAP1 Primärantikörper
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- Applikationshinweise
- WB 1:500-1:2000 , IHC 1:50-1:200
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freeze/thaw cycles.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20°C.
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- Target
- KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))
- Andere Bezeichnung
- KEAP1 (KEAP1 Produkte)
- Synonyme
- INrf2 antikoerper, KLHL19 antikoerper, INRF2 antikoerper, mKIAA0132 antikoerper, Inrf2 antikoerper, fb36f11 antikoerper, keap1 antikoerper, wu:fb36f11 antikoerper, KEAP1 antikoerper, klhl19 antikoerper, zgc:171654 antikoerper, kelch like ECH associated protein 1 antikoerper, kelch-like ECH-associated protein 1 antikoerper, Kelch-like ECH-associated protein 1 antikoerper, kelch-like ECH-associated protein 1a antikoerper, kelch like ECH associated protein 1 L homeolog antikoerper, kelch-like ECH-associated protein 1b antikoerper, KEAP1 antikoerper, Keap1 antikoerper, keap1a antikoerper, keap1 antikoerper, keap1.L antikoerper, keap1b antikoerper
- Gen-ID
- 9817
- UniProt
- Q14145
- Pathways
- Maintenance of Protein Location
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