Histone H4 Antikörper

Produktnummer ABIN4903928

Produktdetails anti-Histone H4 Antikörper

Target: Histone H4

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktmerkmale: The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1,2). Histone acetylation occurs mainly on the amino-terminal tail domains of histones H2A (Lys5), H2B (Lys5, 12, 15, and 20), H3 (Lys9, 14, 18, 23, 27, and 56), and H4 (Lys5, 8, 12, and 16) and is important for the regulation of histone deposition, transcriptional activation, DNA replication, recombination, and DNA repair (1-3). Hyper-acetylation of the histone tails neutralizes the positive charge of these domains and is believed to weaken histone-DNA and nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and increasing the accessibility of DNA to various DNA-binding proteins (4,5). In addition, acetylation of specific lysine residues creates docking sites for a protein module called the bromodomain, which binds to acetylated lysine residues (6). Many transcription and chromatin regulatory proteins contain bromodomains and may be recruited to gene promoters, in part, through binding of acetylated histone tails. Histone acetylation is mediated by histone acetyltransferases (HATs), such as CBP/p300, GCN5L2, PCAF, and Tip60, which are recruited to genes by DNA-bound protein factors to facilitate transcriptional activation (3). Deacetylation, which is mediated by histone deacetylases (HDAC and sirtuin proteins), reverses the effects of acetylation and generally facilitates transcriptional repression (7,8). Histone H4 lysine 5 is acetylated by multiple HAT proteins. Acetylation by Esa1p in yeast, or Tip60 in mammalian cells, may contribute to both transcriptional activation and DNA repair, including non-homologous end joining and replication-coupled repair (9-12).

Aufreinigung: Affinity purification

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF 1:20-1:100, IP 1:20-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C.

Details zu Histone H4

Target: Histone H4

Abstract: Histone H4 Produkte

Synonyme: hist1h4l antikoerper, H1ft antikoerper, Hist4 antikoerper, Hist4h4 antikoerper, H4 antikoerper, X04652 antikoerper, FO108 antikoerper, H4/n antikoerper, H4F2 antikoerper, H4FN antikoerper, HIST2H4 antikoerper, H4C13 antikoerper, H4C7 antikoerper, F8N16.2 antikoerper, F8N16_2 antikoerper, histone H4 antikoerper, BcDNA:RH52884 antikoerper, CG3379 antikoerper, Dmel\CG3379 antikoerper, H4r antikoerper, His4-88CD antikoerper, His4R antikoerper, histone cluster 1, H4a L homeolog antikoerper, histone cluster 1 H4 family member B antikoerper, histone cluster 2, H4 antikoerper, histone cluster 2 H4 family member a antikoerper, histone H4 antikoerper, Histone H4 replacement antikoerper, Histone H4 antikoerper, hist1h4a.L antikoerper, Hist1h4b antikoerper, Hist2h4 antikoerper, HIST2H4A antikoerper, H4 antikoerper, H4C14 antikoerper, HIS4 antikoerper, HIST2H4 antikoerper, His4r antikoerper, his-38 antikoerper

Gen-ID: 121504

UniProt: P62805