2B4 Antikörper (Internal Region, pTyr271)

Produktnummer ABIN487961

Produktdetails anti-2B4 Antikörper

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Bindungsspezifität: Internal Region, pTyr271

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser 2B4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequenz: c-EFLTIPYEDV KD

Spezifität: This antibody recognizes CD244.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_057466.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/64000. Western blot: Approx 75 kDa band observed in Human Natural Killer (NK) cell lysates afterimmunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose(calculated MW of 41.5 kDa according to NP_057466.1). We do note that CD244 is aglycoprotein which makes a higher size band to be expected. Recommended concentration: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu 2B4

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Andere Bezeichnung: CD244 (CD244 Produkte)

Synonyme: CD244 antikoerper, 2B4 antikoerper, NAIL antikoerper, NKR2B4 antikoerper, Nmrk antikoerper, SLAMF4 antikoerper, C9.1 antikoerper, F730046O15Rik antikoerper, Ly90 antikoerper, CD244 molecule antikoerper, CD244 natural killer cell receptor 2B4 antikoerper, CD244 antikoerper, Cd244 antikoerper

Hintergrund: CD244 is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. CD244 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness. Although there have been several molecules shown to interact with CD244, the signaling mechanism ofCD244-mediated activation of NK cells is still unknown. It is a high affinity receptor for CD48.Synonyms: NAIL, NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, NKR2B4, Natural killer cell receptor 2B4

Gen-ID: 51744

NCBI Accession: NP_057466

UniProt: Q9BZW8