PDGF-AA Homodimer Antikörper (Biotin)

Produktnummer ABIN465592

Produktdetails

Target: PDGF-AA Homodimer

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), ELISA

Spezifität: Human PDGF-AA

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human PDGF-AA.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hPDGF-AA by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-AA. To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human PDGF-AA (LS-B7488) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Antigendetails

Target: PDGF-AA Homodimer

Andere Bezeichnung: PDGF-AA

Synonyme: PDGF-A antikoerper, PDGF1 antikoerper, AA409063 antikoerper, CD142 antikoerper, Cf-3 antikoerper, Cf3 antikoerper, TF antikoerper, PDGFACP antikoerper, platelet derived growth factor subunit A antikoerper, coagulation factor III antikoerper, platelet derived growth factor, alpha antikoerper, PDGFA antikoerper, F3 antikoerper, Pdgfa antikoerper

Hintergrund: Name/Gene ID: PDGFA
Family: PDGF

Synonyms: PDGFA, PDGF-A, PDGF A-chain, PDGF subunit A, PDGF-1, PDGF1

Gen-ID: 5154

UniProt: P04085