GDF3 Antikörper

Produktnummer ABIN465020

Produktdetails anti-GDF3 Antikörper

Target: GDF3 (Growth Differentiation Factor 3 (GDF3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GDF3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Human GDF-3

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human GDF-3.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: To detect hGDF-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GDF-3 (LS-C104295) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGDF-3. Western Blot: To detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDF-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu GDF3

Target: GDF3 (Growth Differentiation Factor 3 (GDF3))

Andere Bezeichnung: GDF3 (GDF3 Produkte)

Synonyme: KFS3 antikoerper, MCOP7 antikoerper, MCOPCB6 antikoerper, C78318 antikoerper, Gdf-3 antikoerper, Vgr-2 antikoerper, Vgr2 antikoerper, ecat9 antikoerper, RGD1564178 antikoerper, CVG1 antikoerper, der antikoerper, derriere antikoerper, gdf3-a antikoerper, vgr-2 antikoerper, vgr2 antikoerper, growth differentiation factor 3 antikoerper, growth differentiation factor 3 S homeolog antikoerper, GDF3 antikoerper, Gdf3 antikoerper, gdf3.S antikoerper

Hintergrund: Name/Gene ID: GDF3
Family: TGF beta

Synonyms: GDF3, KFS3, MCOPCB6, GDF-3, MCOP7

Gen-ID: 9573

UniProt: Q9NR23