MIA2 Antikörper

Produktnummer ABIN464847

Produktdetails anti-MIA2 Antikörper

Target: MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MIA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Human MIA-2

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIA-2.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary. ELISA: To detect hMIA-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIA-2 (LS-C104686) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIA-2. Western Blot: To detect hMIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu MIA2

Target: MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))

Andere Bezeichnung: MIA2 (MIA2 Produkte)

Synonyme: MEA6 antikoerper, MGEA antikoerper, MGEA11 antikoerper, MGEA6 antikoerper, zgc:56047 antikoerper, melanoma inhibitory activity 2 antikoerper, CTAGE family, member 5 antikoerper, CTAGE family member 5, ER export factor antikoerper, MIA2 antikoerper, ctage5 antikoerper, CTAGE5 antikoerper, Mia2 antikoerper

Hintergrund: Name/Gene ID: MIA2
Family: MIA

Synonyms: MIA2, Melanoma inhibitory activity 2

Gen-ID: 117153

UniProt: Q96PC5