GNAO1 Antikörper (Subunit alpha)

Produktnummer ABIN462341

Produktdetails anti-GNAO1 Antikörper

Target: GNAO1 (Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O (GNAO1))

Bindungsspezifität: AA 345-354, Subunit alpha

Reaktivität: Maus, Drosophila melanogaster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GNAO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40kD forming a heterotrimeric protein complex with beta and gamma subunits. Two isoforms of the alpha subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2. Has been used successfully for the detection of G(o) in Drosophila by western blotting.

Aufreinigung: Ammonium sulfate precipitation

Immunogen: Synthetic peptide from mouse GNAO1 / GNAO.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:500 - 1:1000), IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: This antibody detects band at 39 kD when used under reducing conditions against rat brain.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu GNAO1

Target: GNAO1 (Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O (GNAO1))

Andere Bezeichnung: GNAO1 (GNAO1 Produkte)

Synonyme: G-ALPHA-o antikoerper, GNAO antikoerper, AW050213 antikoerper, Galphao antikoerper, Gnao antikoerper, alphaO antikoerper, gnao1 antikoerper, wu:fq26h05 antikoerper, zgc:73315 antikoerper, RATBPGTPC antikoerper, XGalpha01 antikoerper, gnao1-A antikoerper, zgc:73153 antikoerper, G protein subunit alpha o1 antikoerper, guanine nucleotide binding protein, alpha O antikoerper, guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O, a antikoerper, G protein subunit alpha o1 L homeolog antikoerper, Guanine nucleotide-binding protein G(o) subunit alpha antikoerper, guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O, b antikoerper, GNAO1 antikoerper, Gnao1 antikoerper, gnao1a antikoerper, gnao1.L antikoerper, goa-1 antikoerper, gnao1b antikoerper

Hintergrund: Name/Gene ID: GNAO1

Synonyms: GNAO1, GNAO, G-ALPHA-o, G0 Protein Alpha

Gen-ID: 2775

UniProt: P09471

Pathways: G-protein mediated Events