PPP4C Antikörper (AA 290-301)

Produktnummer ABIN462300

Produktdetails anti-PPP4C Antikörper

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Bindungsspezifität: AA 290-301

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Pferd, Zebrafisch (Danio rerio), Fledermaus, Hamster, Affe, Xenopus laevis

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PPP4C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human PPP4C.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus, Xenopus, Catfish, Stickleback, Pufferfish, Zebrafish, Water flea (100%) Drosophila, Mosquito, Ant, Beetle (92%) Seq squirt, Poplar, Trichoplax, Toxoplasma, Tick, Spikemoss, Amoeba, Neospora (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-PQETRGIPSKKP from the C-terminus of human PPP4C (NP_002711.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus, Xenopus, Catfish, Stickleback, Pufferfish, Zebrafish, Water flea (100%), Drosophila, Mosquito, Ant, Beetle (92%), Seq squirt, Poplar, Trichoplax, Toxoplasma, Tick, Spikemoss, Amoeba, Neospora (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA, WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot: Approximately 35kD band observed in HEK293, A431 and HeLa lysates (calculated MW of 35.1kD according to NP_002711.1).

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PPP4C

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Andere Bezeichnung: PPP4C (PPP4C Produkte)

Synonyme: pp4 antikoerper, ppx antikoerper, pp4c antikoerper, pph3 antikoerper, MGC75928 antikoerper, PP4C-A antikoerper, gc:56413 antikoerper, ppp4c antikoerper, wu:fd05e08 antikoerper, zgc:56413 antikoerper, PP4 antikoerper, PP4C antikoerper, PPH3 antikoerper, PPP4 antikoerper, PPX antikoerper, 1110002D08Rik antikoerper, AU016079 antikoerper, Ppx antikoerper, protein phosphatase 4 catalytic subunit antikoerper, protein phosphatase 4, catalytic subunit a antikoerper, protein phosphatase 4, catalytic subunit antikoerper, protein phosphatase 4 catalytic subunit S homeolog antikoerper, ppp4c antikoerper, ppp4ca antikoerper, PPP4C antikoerper, Ppp4c antikoerper, ppp4c.S antikoerper

Hintergrund: Name/Gene ID: PPP4C
Subfamily: Protein Phosphatase - Serine/Threonine other
Family: Protein Phosphatase

Synonyms: PPP4C, PP4, PPH3, Protein phosphatase X, PP-X, Protein phosphatase 4, PP4C, PPP4, PPX

Gen-ID: 5531

NCBI Accession: NP_002711

UniProt: P60510