GABRA4 Antikörper (AA 349-361)

Produktnummer ABIN462297

Produktdetails anti-GABRA4 Antikörper

Target: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Bindungsspezifität: AA 349-361

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GABRA4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human GABRA4.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Elephant, Bovine (92%) Rabbit, Opossum (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-EKAKRKTSKPPQE from an internal region of human GABRA4 (NP_000800.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Elephant, Bovine (92%), Rabbit, Opossum (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 70kD band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 61.6kD according to NP_000800.2). Recommended concentration: 0.1-0.3 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GABRA4

Target: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Andere Bezeichnung: GABRA4 (GABRA4 Produkte)

Synonyme: GABRA4 antikoerper, Gabra-4 antikoerper, gamma-aminobutyric acid type A receptor alpha4 subunit antikoerper, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4 antikoerper, GABRA4 antikoerper, Gabra4 antikoerper

Hintergrund: Name/Gene ID: GABRA4
Subfamily: GABA A receptor
Family: Ion Channel

Synonyms: GABRA4, GABA A receptor alpha 4, GABA(A) receptor, alpha 4

Gen-ID: 2557

NCBI Accession: NP_000800

UniProt: P48169

Pathways: Synaptic Membrane