MYL2 Antikörper (AA 1-166)

Produktnummer ABIN462270

Produktdetails anti-MYL2 Antikörper

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Bindungsspezifität: AA 1-166

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MYL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human MYL2 (1-166aa)

Aufreinigung: Protein G purified

Immunogen: Recombinant human MYL2 (1-166aa) purified from E. coli

Type of Immunogen: Recombinant protein

Klon: AT3B2

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Extracts of Rat heart (60 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Details zu MYL2

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Andere Bezeichnung: MYL2 (MYL2 Produkte)

Synonyme: CMH10 antikoerper, MLC2 antikoerper, 2410014J02Rik antikoerper, MLC-2 antikoerper, Mlc2 antikoerper, MLC-2v antikoerper, Mlc2v antikoerper, Mylpc antikoerper, Mlc-2 antikoerper, myl2 antikoerper, zgc:136848 antikoerper, XMLC2 antikoerper, cmh10 antikoerper, mlc2 antikoerper, G2 antikoerper, MLC-2A antikoerper, MLC2V antikoerper, myosin light chain 2 antikoerper, myosin light chain, phosphorylatable, fast skeletal muscle antikoerper, myosin, light polypeptide 2, regulatory, cardiac, slow antikoerper, myosin, light chain 2b, regulatory, cardiac, slow antikoerper, myosin light chain 2 S homeolog antikoerper, myosin, light chain 2, regulatory, cardiac, slow antikoerper, MYL2 antikoerper, Mylpf antikoerper, Myl2 antikoerper, myl2b antikoerper, myl2.S antikoerper

Hintergrund: Name/Gene ID: MYL2

Synonyms: MYL2, MLC2, Myosin light chain 2, MLC-2, RLC of myosin, CMH10, MLC-2v

Gen-ID: 4633

UniProt: P10916

Pathways: Myometrial Relaxation and Contraction