ABCA5 Antikörper (AA 1296-1345)
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- Target Alle ABCA5 Antikörper anzeigen
- ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))
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Bindungsspezifität
- AA 1296-1345
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Reaktivität
- Human, Hund, Kaninchen, Affe
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser ABCA5 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human ABCA5
- Homologie
- Percent identity by BLAST analysis: Human (100%) Mouse, Rat (92%) Dog, Rabbit (85%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa1296-1345 of human ABCA5 (Q8WWZ7, NP_061142). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%), Galago, Mouse, Rat, Panda (92%), Bat (90%), Elephant, Dog, Rabbit, Horse, Opossum (85%).
Type of Immunogen: Synthetic peptide - Isotyp
- IgG
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- Applikationshinweise
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Distilled Water.
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))
- Andere Bezeichnung
- ABCA5 (ABCA5 Produkte)
- Synonyme
- zgc:163009 antikoerper, ABC13 antikoerper, EST90625 antikoerper, B930033A02Rik antikoerper, mKIAA1888 antikoerper, ATP binding cassette subfamily A member 5 antikoerper, ATP-binding cassette, sub-family A (ABC1), member 5 antikoerper, ATP-binding cassette sub-family A member 5 antikoerper, ABCA5 antikoerper, abca5 antikoerper, LOC100545445 antikoerper, Abca5 antikoerper
- Hintergrund
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Name/Gene ID: ABCA5
Subfamily: ATP-binding cassette - ABCA/ABC1
Family: Transporter
Synonyms: ABCA5, ATP-binding cassette A5, ABC13, EST90625, KIAA1888 - Gen-ID
- 23461
- NCBI Accession
- NP_061142
- UniProt
- Q8WWZ7
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