GPX4 Antikörper (AA 184-196)

Produktnummer ABIN461940

Produktdetails anti-GPX4 Antikörper

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Bindungsspezifität: AA 184-196

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GPX4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human GPX4. This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Goat, Hamster, Bovine (92%) Marmoset, Mouse, Rat, Pig, Opossum, Guinea pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-EEPLVIEKDLPHY from the C-terminus of human GPX4 (NP_002076.2, NP_001034937.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Goat, Hamster, Bovine (92%), Marmoset, Mouse, Rat, Pig, Opossum, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (1:16000, 0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Andere Bezeichnung: GPX4 / MCSP (GPX4 Produkte)

Synonyme: GPx-4 antikoerper, GSHPx-4 antikoerper, MCSP antikoerper, PHGPx antikoerper, snGPx antikoerper, snPHGPx antikoerper, Phgpx antikoerper, gpx-4 antikoerper, snGpx antikoerper, cb563 antikoerper, cb691 antikoerper, PHGPxa antikoerper, sb:cb563 antikoerper, zgc:92253 antikoerper, wu:fb82e03 antikoerper, 1700027O09Rik antikoerper, mtPHGPx antikoerper, ATGPX4 antikoerper, F11L15.5 antikoerper, glutathione peroxidase 4 antikoerper, GP-GPx4 antikoerper, glutathione peroxidase 4 antikoerper, glutathione peroxidase 4a antikoerper, GPX4 antikoerper, Gpx4 antikoerper, gpx4a antikoerper

Hintergrund: Name/Gene ID: GPX4

Synonyms: GPX4, GPx-4, MCSP, SnPHGPx, Phospholipid hydroperoxidase, SnGPx, Glutathione peroxidase 4, GSHPx-4, PHGPx

Gen-ID: 2879

NCBI Accession: NP_002076, NP_001034937

UniProt: P36969