IREB2 Antikörper (Internal Region)

Produktnummer ABIN453972

Produktdetails anti-IREB2 Antikörper

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Bindungsspezifität: Internal Region

Reaktivität: Human, Ratte, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IREB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: c-SIHYEGSEYK LSHGS

Spezifität: This antibody recognizes Iron Regulatory Protein 2 (IREB2/IRP2).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of IREB2 (IRP2) according to NP_004127.1. Genename: IREB2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: IREB2 Antibody Detection limit dilution 1/32000. Western blot: 1-3 μg/mL. Approx 110 kDa band observed in Human Liver lysates (calculatedMW of 100 kDa according to NP_004127.1). An additional band of unknown identity wasalso consistently observed at 60 kDa. This band was successfully blocked by incubationwith the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu IREB2

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Andere Bezeichnung: IREB2 (IREB2 Produkte)

Synonyme: IREB2 antikoerper, im:7153062 antikoerper, DKFZp468N047 antikoerper, D9Ertd85e antikoerper, Irp2 antikoerper, ACO3 antikoerper, IRP2 antikoerper, IRP2AD antikoerper, IREBP2 antikoerper, iron responsive element binding protein 2 antikoerper, iron-responsive element binding protein 2 antikoerper, iron responsive element binding protein 2 L homeolog antikoerper, IREB2 antikoerper, ireb2 antikoerper, Ireb2 antikoerper, ireb2.L antikoerper

Hintergrund: Iron regulatory protein 2 (IREB2 or IRP2) is a central regulator of cellular iron homeostasis due to its regulation of specific mRNAs encoding proteins of iron uptake and storage. In mammalian cells, regulation of the expression of proteins involved in iron metabolism is achieved through interactions of iron-sensing proteins known as iron regulatory proteins (IRPs), with transcripts that contain RNA stem-loop structures referred to as iron responsive elements (IREs). Two distinct but highly homologous proteins, IRP1 and IRP2, bind IREs with high affinity when cells are depleted of iron, inhibiting translation of some transcripts, such as ferritin, or turnover of others, such as the transferrin receptor (TFRC). IRPs sense cytosolic iron levels and modify expression of proteins involved in iron uptake, export and sequestration according to the needs of individual cells. Regulating the amount of iron sequestered by ferritin and taken up by transferrin receptor ensures that cells acquire sufficient iron for their needs, while preventing iron toxicity.Synonyms: IRE-BP2, IRP2, Iron regulatory protein 2, Iron-responsive element-binding protein 2

Gen-ID: 3658

NCBI Accession: NP_004127

UniProt: P48200

Pathways: Transition Metal Ion Homeostasis