TRPM7 Antikörper (C-Term)

Produktnummer ABIN452417

Produktdetails anti-TRPM7 Antikörper

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Bindungsspezifität: AA 1817-1863, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TRPM7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects TRPM7 at ~220 kDa. Shows no cross reactivity with TrpM6.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat.

Produktmerkmale: Synonyms: CHAK1, LTRPC7, Transient receptor potential cation channel subfamily M member 7,Channel-kinase 1, Long transient receptor potential channel 7

Aufreinigung: Protein G Chromatography

Immunogen: Fusion protein amino acids 1817-1863 (cytoplasmic C-terminus) of mouse TrpM7

Klon: S74-25

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 1-10 μg/mL (if results are off, try using the lysate without boiling). Immunoprecipitation. Immunocytochemistry: 0.1-1.0 μg/mL (Perox).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 50 % Glycerol and 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu TRPM7

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Andere Bezeichnung: TRPM7 (TRPM7 Produkte)

Synonyme: cb495 antikoerper, TRPM7 antikoerper, chak antikoerper, chak1 antikoerper, ltrpc7 antikoerper, trp-plik antikoerper, ALSPDC antikoerper, CHAK antikoerper, CHAK1 antikoerper, LTRPC7 antikoerper, TRP-PLIK antikoerper, 2310022G15Rik antikoerper, 4833414K03Rik antikoerper, 5033407O22Rik antikoerper, LTrpC-7 antikoerper, Ltpr7 antikoerper, Ltrpc7 antikoerper, TRPPLIK antikoerper, Chak antikoerper, Trp-plik antikoerper, transient receptor potential cation channel subfamily M member 7 antikoerper, transient receptor potential cation channel, subfamily M, member 7 antikoerper, TRPM7 antikoerper, trpm7 antikoerper, Trpm7 antikoerper

Hintergrund: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants (3, 4).Synonyms: CHAK1, Channel-kinase 1, LTRPC7, Long transient receptor potential channel 7, Transient receptor potential cation channel subfamily M member 7

Gen-ID: 58800

UniProt: Q923J1