NRIP1 Antikörper (Internal Region)

Produktnummer ABIN401524

Produktdetails anti-NRIP1 Antikörper

Target: NRIP1 (Nuclear Receptor Interacting Protein 1 (NRIP1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NRIP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequenz: c-EKDSPRLTKT NP

Spezifität: This antibody detects RIP140 / NRIP1 (Internal).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_003480.2 . Genename: NRIP1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 140 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 120 kDa according to NP_003480.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu NRIP1

Target: NRIP1 (Nuclear Receptor Interacting Protein 1 (NRIP1))

Andere Bezeichnung: NRIP1 (NRIP1 Produkte)

Synonyme: MGC68665 antikoerper, NRIP1 antikoerper, RIP140 antikoerper, 6030458L20Rik antikoerper, 8430438I05Rik antikoerper, 9630050P12 antikoerper, AA959574 antikoerper, AW456757 antikoerper, nuclear receptor interacting protein 1 S homeolog antikoerper, nuclear receptor interacting protein 1 antikoerper, circumsporozoite protein antikoerper, nrip1.S antikoerper, NRIP1 antikoerper, nrip1 antikoerper, Nrip1 antikoerper, LOC100627733 antikoerper

Hintergrund: RIP140 is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as nuclear receptor interacting protein 1 (NRIP1), this protein modulates transcriptional activity of the estrogen receptor. Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Receptor Interacting Protein 140 (RIP 140) has been shown to enhance estrogen receptor transcriptional activity through the AF2 activation domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP 140 has recently been shown to interact with peroxisome proliferator-activated receptor-alpha (PPAR alpha) and has been proposed to competitively inhibit PPAR alpha interaction with Steroid Receptor Coactivator-1 (SRC-1).Synonyms: Nuclear factor RIP140, Nuclear receptor-interacting protein 1, Receptor-interacting protein 140

Gen-ID: 8204

NCBI Accession: NP_003480

UniProt: P48552

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway