CLIP1 Antikörper (C-Term)

Produktnummer ABIN401523

Produktdetails anti-CLIP1 Antikörper

Target: CLIP1 (CAP-GLY Domain Containing Linker Protein 1 (CLIP1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CLIP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: c-PHSTHHGSRG EERP

Spezifität: This antibody detects CLIP1 / Restin at C-term. It is expected to recognize both reported isoforms (NP_002947.1, NP_937883.1).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Dog, PigSpecies reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C-Terminus of the protein sequence according to NP_002947.1, NP_937883.1 . Genename: CLIP1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/8000. Western blot: Approx 160 kDa band observed in Human Breast Cancer lysates (calculatedMW of 160 kDa according to NP_002947.1. Recommended concentration: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: Human Thyroid Gland shows structuredcytoplasm staining in active epithelial cells. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu CLIP1

Target: CLIP1 (CAP-GLY Domain Containing Linker Protein 1 (CLIP1))

Andere Bezeichnung: CLIP1 / Restin (CLIP1 Produkte)

Synonyme: RSN antikoerper, CLIP1 antikoerper, clip170 antikoerper, clip-170 antikoerper, CLIP antikoerper, CLIP-170 antikoerper, CLIP170 antikoerper, CYLN1 antikoerper, 1110007I12Rik antikoerper, 4631429H07Rik antikoerper, AV017631 antikoerper, C81039 antikoerper, Clip50 antikoerper, Rsn antikoerper, mKIAA4046 antikoerper, restin antikoerper, CAP-Gly domain containing linker protein 1 antikoerper, CAP-Gly domain-containing linker protein 1 antikoerper, CAP-Gly domain containing linker protein 1 L homeolog antikoerper, CAP-GLY domain containing linker protein 1b antikoerper, CAP-GLY domain containing linker protein 1 antikoerper, CLIP1 antikoerper, LOC100075022 antikoerper, clip1 antikoerper, clip1.L antikoerper, clip1b antikoerper, Clip1 antikoerper

Hintergrund: CLIP170 associates with the growing ends of microtubules, a type of behaviour known as 'plus end tracking' that is exhibited by proteins called +TIPS. It is known to bind to CLASPS and IQGAP1, and is thought to play a role as a positive regulator of microtubule dynamics. CLIP170 is also involved in targeting of the dynactin complex to the ends of growing microtubules, and may be important in mitosis.Synonyms: CAP-Gly domain-containing linker protein 1, CLIP-170, CYLN1, Cytoplasmic linker protein 1, Cytoplasmic linker protein 170 alpha-2, RSN, Reed-Sternberg intermediate filament-associated protein

Gen-ID: 6249

NCBI Accession: NP_002947

UniProt: P30622

Pathways: Microtubule Dynamics