HHV8 Antikörper (C-Term)

Produktnummer ABIN401445

Produktdetails anti-HHV8 Antikörper

Target: HHV8 (Herpes Virus Type 8 (HHV8))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HHV8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/100,000 - 1/170,000. Western Blot: 1/5,000 - 1/8,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.55 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu HHV8

Target: HHV8 (Herpes Virus Type 8 (HHV8))

Andere Bezeichnung: Herpes Virus Type 8 / HHV8 (HHV8 Produkte)

Substanzklasse: Virus

Hintergrund: ORF57 (also known as MTA), one of the earliest Kaposi's sarcoma-associated herpesvirus (KSHV) regulatory proteins to be expressed, is essential for virus lytic replication. A counterpart is present in every herpesvirus sequenced, indicating the importance of this signature viral protein, and those examined act post-transcriptionally, affecting RNA splicing and transport. KSHV ORF57 is capable of establishing both lytic and latent replication cycles. In KS, the virus localizes to tumor progenitor endothelial cells, most of which are latently infected. In cell culture, KSHV replication is generally studied using B-cell lines, such as BCBL-1, generated from primary effusion lymphoma material. Most BCBL-1 cells are latently infected, although there is some spontaneous virus reactivation. Addition of chemical inducers such as sodium n-butyrate, 12-O-tetradecanoylphorbol-13-acetate (TPA), and valproic acid (VA) to these cells efficiently induces the lytic cycle and produces virions. KSHV ORF57 protein predominantly localizes to the nucleus and can shuttle between the nucleus and cytoplasm. Most HSV-1 genes are unspliced, by contrast, ORF57 is spliced gene, the protein is 455 amino acids in length and 50 kDa in size.Synonyms: HHV-8, KHSV