MYL6 Antikörper (AA 12-27)

Produktnummer ABIN401347

Produktdetails anti-MYL6 Antikörper

Target: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Bindungsspezifität: AA 12-27

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MYL6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against the regulatory light chain of smooth and nonmuscle Myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 12-27 of Human Myosin light chain protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Tested in ELISA (1/5000-1/20000) and Western Blot (1/500-1/2000). Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation (1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.71 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu MYL6

Target: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Andere Bezeichnung: MYL6 (MYL6 Produkte)

Synonyme: ESMLC antikoerper, LC17 antikoerper, LC17-GI antikoerper, LC17-NM antikoerper, LC17A antikoerper, LC17B antikoerper, MLC-3 antikoerper, MLC1SM antikoerper, MLC3NM antikoerper, MLC3SM antikoerper, zgc:103624 antikoerper, MLC3nm antikoerper, Myln antikoerper, myosin light chain 6 antikoerper, myosin, light chain 6, alkali, smooth muscle and non-muscle antikoerper, myosin light chain 6 L homeolog antikoerper, myosin, light polypeptide 6, alkali, smooth muscle and non-muscle antikoerper, MYL6 antikoerper, Myl6 antikoerper, myl6 antikoerper, myl6.L antikoerper

Hintergrund: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, mouse and rat have almost identical sequences.Synonyms: 17 kDa myosin light chain, LC17, MLC-3, Myosin light chain 3, Myosin light chain alkali 3, Myosin light polypeptide 6, Smooth muscle and nonmuscle myosin light chain alkali 6

Gen-ID: 4637

NCBI Accession: NP_066299

UniProt: P60660