FITC Antikörper (HRP)

Produktnummer ABIN401127

Produktdetails anti-FITC Antikörper (HRP)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reaktivität: Bitte anfragen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FITC Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Dot Blot (DB)

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Fluorescein conjugated to Goat IgG

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electronmicroscopy and immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/2,000-1/5,000. Immunohistochemistry: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL of deionized water (or equivalent).

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (IgG and Protease Free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Do Not Add Sodium Azide

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Referenzen für anti-FITC Antikörper (HRP) (ABIN401127)

Tokuji, Maeda, Yorimitsu, Osuka: "New synthetic strategy for diporphyrins: pinacol coupling-rearrangement." in: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 17, Issue 26, pp. 7154-7, (2011) (PubMed).

Hamzah, Kotamraju, Seo, Agemy, Fogal, Mahakian, Peters, Roth, Gagnon, Ferrara, Ruoslahti: "Specific penetration and accumulation of a homing peptide within atherosclerotic plaques of apolipoprotein E-deficient mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 17, pp. 7154-9, (2011) (PubMed).

Details zu FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Andere Bezeichnung: FITC (FITC Produkte)

Substanzklasse: Chemical

Hintergrund: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate