GPC1 Antikörper (Internal Region)

Produktnummer ABIN401058

Produktdetails anti-GPC1 Antikörper

Target: GPC1 (Glypican 1 (GPC1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte, Hund, Makake, Opossum

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody is specific for Glypican-1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Macaque, Canine, Cattle, Opossum.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide corresponding to an internal region of Human Glypican-1 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 2,500 - 1: 3000. Western Blot: 1: 800 - 1: 1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 2.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu GPC1

Target: GPC1 (Glypican 1 (GPC1))

Andere Bezeichnung: Glypican-1 / GPC1 (GPC1 Produkte)

Synonyme: gpc1 antikoerper, zgc:63947 antikoerper, GPC1 antikoerper, Glypican-1 antikoerper, glypican antikoerper, AI462976 antikoerper, HSPG M12 antikoerper, HSPGM12 antikoerper, glypican 1b antikoerper, glypican 1 antikoerper, gpc1b antikoerper, GPC1 antikoerper, gpc1 antikoerper, Gpc1 antikoerper

Hintergrund: Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary from 17 % to 63 % identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican-1 has been shown to interact with SLIT2.

Gen-ID: 2817

NCBI Accession: NP_002072

UniProt: P35052

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation