Keratin 7/17 Antikörper

Produktnummer ABIN392948

Produktdetails anti-Keratin 7/17 Antikörper

Target: Keratin 7/17 (KRT7/17)

Reaktivität: Human, Rind (Kuh), Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 7/17 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Recognizes a unique epitope shared by the intermediate filament keratins, cytokeratin 7 and cytokeratin 17, a 54kD type II basic cytokeratin, and 46kD type I acidic cytokeratin respectively. Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissues and their neoplasms, including the lungs, ovary and breast. Each keratin filament consists of at least one type I and one type II keratin chain, which then assemble to form a cytoskeletal network, extending from the surface of the nucleus to the cell membrane. The expression of a particular type of cytokeratin depends mainly on the type of epithelium and the development stage of the cell, including its corresponding malignant state. Immunohistochemical analysis of cytokeratin profiles is therefore a valuable tool for diagnosis and characterization, and this particular antibody could be useful for the evaluation of some stratified epithelia which express cytokeratin 17, but not cytokeratin 7. Reported as suitable for use in immunocytochemistry.

Keine Kreuzreaktivität: Maus, Kaninchen, Ratte (Rattus)

Aufreinigung: Affinity purified

Immunogen: HeLa cell cytoskeleton preparation.

Klon: C-46

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (20 - 30 μg/mL), IP, WB (2 - 3 μg/mL)

Usage: Western Blot: LS-C58267 detects bands of approximately 54kD and 46kD in HeLa cytoskeleton cell lysates under reducing conditions. Immunohistology: This product requires protein digestion pre-treatment of paraffin sections using pepsin prior to staining.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Short term: -20°C
Long term: -20°C.

Details zu Keratin 7/17

Target: Keratin 7/17 (KRT7/17)

Andere Bezeichnung: Cytokeratin 7+17 (KRT7/17 Produkte)

Synonyme: KRT7 antikoerper, CK7 antikoerper, keratin 7 antikoerper, KRT7 antikoerper

Hintergrund: This antibody detects: Cytokeratin 7+17