RAF1 Antikörper (pSer43)

Produktnummer ABIN389730

Dieses Anti-RAF1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RAF1 in DB. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für RAF1 Antikörper (pSer43) (ABIN389730)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAF1 Antikörper ist unkonjugiert

Applikation: Dot Blot (DB)

Klon: RB11127

Produktdetails anti-RAF1 Antikörper

Bindungsspezifität: pSer43

Homologie: B, M, Rat

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This RAF1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S43 of human RAF1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: DB: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-RAF1 Antikörper (ABIN389730)

Kamemura, Murakami, Komatsu, Sawanoi, Miyamoto, Ishidoh, Kishimoto, Tsuji, Yuasa: "Type II cGMP-dependent protein kinase negatively regulates fibroblast growth factor signaling by phosphorylating Raf-1 at serine 43 in rat chondrosarcoma cells." in: Biochemical and biophysical research communications, Vol. 483, Issue 1, pp. 82-87, (2017) (PubMed).

Details zu RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Andere Bezeichnung: RAF1

Hintergrund: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Molekulargewicht: 73052

Gen-ID: 5894

NCBI Accession: NP_002871

UniProt: P04049

Pathways: MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling