Arylsulfatase B Antikörper (Internal Region)

Produktnummer ABIN374887

Produktdetails anti-Arylsulfatase B Antikörper

Target: Arylsulfatase B (ARSB)

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Arylsulfatase B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequenz: C-KLARGHTNGT KPLD

Spezifität: This antibody detects internal Arylsulfatase B. It is expected to recognise both reported isoforms (NP_000037.2, NP_942002.1).

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_000037.2, NP_942002.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: limit dilution 1: 64000. Western blot: 0.03-0.1 μg/mL. Approx 45 kDa band observed in Human Heart lysates(calculated MW of 46.5 kDa according to NP_942002.1). An additional band of unknownidentity was also consistently observed at 35 kDa. This band was successfully blocked byincubation with the immunising peptide. We would appreciate any feedback from peoplein the field - have any such results been reported with other antibodies/lysates? Have anyfurther splice variants/modified forms been reported?Immunohistochemistryon paraffin sections: 5-10 μg/mL. In Human Skeletal Muscle showsstriated pattern staining of majority of muscular fibres.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-Arylsulfatase B Antikörper (ABIN374887)

Zhao, Wang, Li, Dong, Wu: "Arylsulfatase B Mediates the Sulfonation-Transport Interplay in Human Embryonic Kidney 293 Cells Overexpressing Sulfotransferase 1A3." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 44, Issue 9, pp. 1441-9, (2018) (PubMed).

Details zu Arylsulfatase B

Target: Arylsulfatase B (ARSB)

Andere Bezeichnung: Arylsulfatase B (ARSB Produkte)

Synonyme: ASB antikoerper, G4S antikoerper, MPS6 antikoerper, 1110007C02Rik antikoerper, AI480648 antikoerper, As-1 antikoerper, As-1r antikoerper, As-1s antikoerper, As-1t antikoerper, As1 antikoerper, As1-r antikoerper, As1-s antikoerper, As1-t antikoerper, Asr-1 antikoerper, Ast-1 antikoerper, ARSB antikoerper, arylsulfatase B antikoerper, arylsulfatase antikoerper, arylsulfatase b antikoerper, ARSB antikoerper, Arsb antikoerper, RB348 antikoerper, LOC5566067 antikoerper, CpipJ_CPIJ011047 antikoerper, VDBG_03275 antikoerper, arsb antikoerper, LOC5579667 antikoerper

Hintergrund: Arylsulfatase B (ARSB) belongs to the sulfatase family. The arylsulfatase B homodimer catalyzes hydrolysis of the sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. Defects in this gene cause Maroteaux-Lamy syndrome.Synonyms: ARSB, ASB, G4S, N-acetylgalactosamine-4-sulfatase

Gen-ID: 411, 9606

UniProt: P15848

Pathways: Glycosaminoglycan Metabolic Process