Neuroligin 2 Antikörper (Internal Region)

Produktnummer ABIN374806

Produktdetails anti-Neuroligin 2 Antikörper

Target: Neuroligin 2 (NLGN2)

Bindungsspezifität: Internal Region

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Neuroligin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: C-NPPDTDIRDP GKK

Spezifität: This antibody detects Neuroligin-2.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Canine (Dog).
Species reactivity (tested):Human, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_065846.1 Genename: NLGN2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 8000. Western blot: Approx 90 kDa band observed in Human Pancreas lysates (calculated MW of90.0 kDa according to NP_065846.1). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 50 kDa. Thisband was successfully blocked by incubation with the immunising peptide. We wouldappreciate any feedback from people in the field - have any such results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Neuroligin 2

Target: Neuroligin 2 (NLGN2)

Andere Bezeichnung: Neuroligin-2 (NLGN2 Produkte)

Synonyme: Nlgn2 antikoerper, GB10066 antikoerper, NLGN2 antikoerper, CCE-L2 antikoerper, NV50031 antikoerper, Nv123771 antikoerper, NL2 antikoerper, RP23-422L16.24 antikoerper, neuroligin 2 antikoerper, LOC410779 antikoerper, NLGN2 antikoerper, LOC663865 antikoerper, LOC100123771 antikoerper, Nlgn2 antikoerper

Hintergrund: Neuroligins are cell adhesion proteins that are thought to instruct the formation and alignment of synaptic specializations. The three known rodent neuroligin isoforms share homologous extracellular acetylcholinesterase-like domains that bridge the synaptic cleft and bind beta-neurexins. All neuroligins have identical intracellular C-terminal motifs that bind to PDZ domains of various target proteins. Neuroligin 2 is exclusively localized to inhibitory synapses in rat brain and dissociated neurons. In immature neurons, neuroligin 2 is found at synapses and also at GABAA receptor aggregates that are not facing presynaptic termini.Synonyms: KIAA1366, NLGN2

Gen-ID: 57555, 9606

UniProt: Q8NFZ4

Pathways: Cell-Cell Junction Organization, Synaptic Membrane