Paired Box 3 Antikörper (N-Term)

Produktnummer ABIN374426

Produktdetails anti-Paired Box 3 Antikörper

Target: Paired Box 3 (PAX3)

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Paired Box 3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: TTLAGAVPRM M-C

Spezifität: This antibody detects PAX3 at N-term. This antibody is expected to recognise all 7 reported human isoforms (as represented by NP_000429.2, NP_039230.1, NP_852122.1,.1 NP_852123.1, NP_852124.1, NP_852125.1 and NP_852126.1).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat and Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_000429.2, NP_039230.1, NP_852122.1,.1 NP_852123.1, NP_852124.1, NP_852125.1, NP_852126.1. Genename: PAX3

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Antibody Limit Dilution: 1/64000. Western blot: Preliminary experiments gave an approx 30-35 kDa band in Human Kidneylysates at 1 μg/mL. Immunohistochemistry: 3 μg/mL. In paraffin embedded Human Oesophagus showsnuclear staining of cells in the Squamous Epithelium.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Details zu Paired Box 3

Target: Paired Box 3 (PAX3)

Andere Bezeichnung: PAX3 (PAX3 Produkte)

Synonyme: PAX3 antikoerper, cdhs antikoerper, hup2 antikoerper, pax-3 antikoerper, ws1 antikoerper, xpax3 antikoerper, Pax3 antikoerper, pax3 antikoerper, pax3b antikoerper, xPax3-B antikoerper, CDHS antikoerper, HUP2 antikoerper, WS1 antikoerper, WS3 antikoerper, Pax-3 antikoerper, Sp antikoerper, splotch antikoerper, zgc:92547 antikoerper, paired box 3 antikoerper, paired box 3 L homeolog antikoerper, paired box 3a antikoerper, PAX3 antikoerper, pax3 antikoerper, pax3.L antikoerper, Pax3 antikoerper, pax3a antikoerper

Hintergrund: This protein is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These proteins play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2,13)(q35,q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.Synonyms: HUP2, Paired box protein Pax-3, Pax-3

Gen-ID: 5077

NCBI Accession: NP_000429

UniProt: P23760

Pathways: Sensory Perception of Sound, Tube Formation