GOPC Antikörper (C-Term)

Produktnummer ABIN374395

Produktdetails anti-GOPC Antikörper

Target: GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))

Bindungsspezifität: C-Term

Reaktivität: Human, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GOPC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Sequenz: C-LDDLHTLYHK KSY

Spezifität: This antibody detects PIST / FIG / GOPC at C-term. It is expected to recognize both reported isoforms.

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog), Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_065132.1, NP_001017408.1 Genename: GOPC

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.05-0.2 μg/mL. Approx.55 kDa band observed in Human Brain (FrontalCortex) lysates (calculated MW of 50.5 kDa according to NP_065132.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GOPC

Target: GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))

Andere Bezeichnung: GOPC / PIST (GOPC Produkte)

Synonyme: DKFZp459H2130 antikoerper, 2210402P09Rik antikoerper, AI844555 antikoerper, CAL antikoerper, FIG antikoerper, GOPC1 antikoerper, PIST antikoerper, fe38f01 antikoerper, wu:fe38f01 antikoerper, zgc:56254 antikoerper, zgc:65853 antikoerper, dJ94G16.2 antikoerper, cal antikoerper, gopc1 antikoerper, pist antikoerper, golgi associated PDZ and coiled-coil motif containing antikoerper, golgi-associated PDZ and coiled-coil motif containing antikoerper, golgi-associated PDZ and coiled-coil motif containing S homeolog antikoerper, GOPC antikoerper, Gopc antikoerper, gopc antikoerper, gopc.S antikoerper

Hintergrund: PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.Synonyms: CAL, CFTR-associated ligand, FIG, Fused in glioblastoma, Golgi-associated PDZ and coiled-coil motif-containing protein, PDZ protein interacting specifically with TC10

Gen-ID: 57120

NCBI Accession: NP_065132

UniProt: Q9HD26

Pathways: Maintenance of Protein Location, Asymmetric Protein Localization