KLF15 Antikörper (N-Term)

Produktnummer ABIN374226

Produktdetails anti-KLF15 Antikörper

Target: KLF15 (Kruppel-Like Factor 15 (KLF15))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KLF15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: VDHLLPVDEN FSS-C

Spezifität: This antibody detects KLF15 at N-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Pig.
Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_054798.1 Genename: KLF15

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.3-1.0 μg/mL. Approx 48 kDa band observed in lysates of hepatoblastomacell line HepG2 (calculated MW of 44.8 kDa according to NP_054798.1). Recommended(Data obtained with a previous EB05463 batch). Identical WB results were obtained with

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu KLF15

Target: KLF15 (Kruppel-Like Factor 15 (KLF15))

Andere Bezeichnung: KLF15 (KLF15 Produkte)

Synonyme: KKLF antikoerper, 1810013I09Rik antikoerper, AV048136 antikoerper, AW494632 antikoerper, CKLF antikoerper, Kruppel like factor 15 antikoerper, Kruppel-like factor 15 S homeolog antikoerper, Kruppel-like factor 15 antikoerper, KLF15 antikoerper, klf15.S antikoerper, Klf15 antikoerper

Hintergrund: The kidney chloride channel proteins CLCNKA and CLCNKB are highly related, although they are located in different areas of the kidney. Using a yeast 1-hybrid screen of a kidney cDNA library with the GA element of the CLCNKA promoter as bait, Uchida et al. (2000) isolated cDNAs encoding MAZ and KLF15, which they termed KKLF. Sequence analysis predicted that the 415-amino acid KLF15 protein, which is 84 % identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. Western blot analysis showed expression of a 50-kD protein. Immunohistochemical analysis detected nuclear expression of KLF15 in liver sinusoid stellate cells, subcapsular fibroblasts, and portal fibroblasts. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.Synonyms: CKLF, Cardiovascular Krueppel-like factor, KKLF, KLF15, Kidney-enriched krueppel-like factor, Krueppel-like factor 15

Gen-ID: 28999

NCBI Accession: NP_054798

UniProt: Q9UIH9