Annexin a1 Antikörper (AA 324-337)

Produktnummer ABIN364419

Produktdetails anti-Annexin a1 Antikörper

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Bindungsspezifität: AA 324-337

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Schwein, Kaninchen, Affe, Rind (Kuh), Pferd, Fledermaus, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Annexin a1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human ANXA1 / Annexin A1.

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Guinea pig (100%) Platypus (93%) Lizard (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide CQAILDETKGDYEK from the C-terminus of human ANXA1 / Annexin A1 (NP_000691.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Guinea pig (100%), Platypus (93%), Lizard (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (4 μg/mL), Peptide-ELISA (1:32000), WB (0.5 - 1.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4 μg/mL. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 35kD band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7kD according to NP_000691.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paweletz et al, Cancer Res. 2000 Nov15,60(22):6293-7. PMID: 11103786). Recommended concentration: 0.5-1.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Andere Bezeichnung: ANXA1 / Annexin A1 (ANXA1 Produkte)

Synonyme: anx1a antikoerper, anxa1 antikoerper, wu:fa01d11 antikoerper, wu:fa05d11 antikoerper, wu:fk69h01 antikoerper, MGC64363 antikoerper, anx1 antikoerper, lpc1 antikoerper, MGC89164 antikoerper, ANXA1 antikoerper, ANX1 antikoerper, LPC1 antikoerper, p35 antikoerper, Anx-1 antikoerper, Anx-A1 antikoerper, C430014K04Rik antikoerper, Lpc-1 antikoerper, Lpc1 antikoerper, Anx1 antikoerper, annexin A1a antikoerper, annexin A1 antikoerper, annexin A1 L homeolog antikoerper, Annexin A1 antikoerper, anxa1a antikoerper, anxa1 antikoerper, anxa1.L antikoerper, ANXA1 antikoerper, LOC100335033 antikoerper, Anxa1 antikoerper

Hintergrund: Name/Gene ID: ANXA1

Synonyms: ANXA1, Annexin A1, Annexin I, Annexin-1, ANX1, Calpactin II, Chromobindin-9, Lipocortin I, Annexin I (lipocortin I), Calpactin-2, LPC1

Gen-ID: 301

NCBI Accession: NP_000691

UniProt: P04083

Pathways: Hormone Transport