Syndecan 2 Antikörper (Internal Region)

Produktnummer ABIN364375

Produktdetails anti-Syndecan 2 Antikörper

Target: Syndecan 2 (SDC2)

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Syndecan 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Recognizes human syndecan-2 (fibroglycan). It recognizes the unglyconated form and may recognize the glyconated form. On Western Blots, it identifies a single band at ~22kD. An additional band at ~40kD may also be observed which may represent a glycanated form. Reactivity has been confirmed with human A-549 lung carcinoma, HEP-G2 hepatocellular carcinoma, HT-29 colorectal adenocarcinoma, K-562 chronic myelogenous leukemia-bone marrow, TF-1 erythroleukemia-bone marrow and U-2 OS osteosarcoma cell lysates.

Aufreinigung: Affinity purified

Immunogen: Synthetic peptide derived from the internal region of human syndecan-2 (fibroglycan).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Syndecan 2

Target: Syndecan 2 (SDC2)

Andere Bezeichnung: SDC2 / Syndecan 2 (SDC2 Produkte)

Synonyme: CD362 antikoerper, HSPG antikoerper, HSPG1 antikoerper, SYND2 antikoerper, 4833414L08Rik antikoerper, AA960457 antikoerper, Hspg1 antikoerper, Synd2 antikoerper, syndecan-2 antikoerper, cb440 antikoerper, zgc:103676 antikoerper, sdc2 antikoerper, SYND2-B antikoerper, Xsyn-2 antikoerper, hspg antikoerper, hspg1 antikoerper, sdc2-a antikoerper, sdc2-b antikoerper, synd2 antikoerper, syndecan 2 antikoerper, syndecan 2 L homeolog antikoerper, syndecan 2 S homeolog antikoerper, SDC2 antikoerper, Sdc2 antikoerper, sdc2 antikoerper, sdc2.L antikoerper, sdc2.S antikoerper

Hintergrund: Name/Gene ID: SDC2

Synonyms: SDC2, CD362, CD362 antigen, HSPG, HSPG1, Fibroglycan, Syndecan proteoglycan 2, SYND2, Syndecan 2, Syndecan-2

Gen-ID: 6383

UniProt: P34741

Pathways: Glycosaminoglycan Metabolic Process