Maus anti-Human IgE (Fc Region) Antikörper

Details zu Produkt Nr. ABIN363691, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Fc Region
164
54
50
8
1
1
1
1
Reaktivität
Human
470
158
52
44
6
6
3
3
2
2
2
1
1
1
Wirt
Maus
296
273
68
68
14
5
3
1
1
Klonalität (Klon)
Monoklonal ()
Applikation
Functional Studies (Func), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen purified human myeloma IgE
Klon Le27-NBS01
Isotyp IgG1 kappa
Spezifität Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes
Produktmerkmale IGHE, chromosome 14q32.33
Reinigung Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
Reinheit > 95 %, tested by analytical Gel Permeation Chromatography
Hintergrund The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.
Applikations-hinweise For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer PBS, pH=7.2, 0.1 % NaN3
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Lagerung 4 °C
Bilder des Herstellers
 image for Mouse anti-Human IgE (Fc Region) antibody (ABIN363691) product purity: gel permeation chromatography (Superose 12/HR)
 image for Mouse anti-Human IgE (Fc Region) antibody (ABIN363691) Target binding kinetics (Biacore), Binding to human IgE (SUS-11)
Produkt verwendet in: Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed).

Allgemeine Veröffentlichungen Knutti-Mueller, Stadler, Magnusson, de Weck: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." in: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).