Dieser Estrogen Receptor alpha Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Immunohistochemistry (IHC)
Spezifität
The antibody detects endogenous level of total Estrogen Receptor-a protein.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Immunogen
Peptide sequence around AA 104-108 (S-P- S-P-L) derived from Human Estrogen Receptor-a. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
ESR1
Reaktivität: Human, Maus, Ratte
WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Yamaguchi, Zhu, Yu, Sasaki, Umetsu, Kidachi, Ryoyama et al.: "Serum-free mouse embryo cells generate a self-sustaining feedback loop for an astrocyte marker protein and respond to cytokines and bisphenol A in accordance with the subtle difference in their ..." in: Cell biology international, Vol. 31, Issue 6, pp. 638-44, (2007) (PubMed).
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.