p53 Antikörper (pSer392)

Produktnummer ABIN361513

Produktdetails anti-p53 Antikörper

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Bindungsspezifität: pSer392

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Specific for the ~53k p53 protein phosphorylated at Ser392. The immunolabeling is completely eliminated by (-phosphatase treatment.

Kreuzreaktivität: Ratte (Rattus)

Homologie: human

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: TP53 (TP53 Produkte)

Hintergrund: P53 has a well established role in blocking the proliferative action of damaged cells and acting in essence as an anticancer agent (Sharpless and DePinho, 2002, Yin et al., 1992). It has been called the guardian of the genome (Lane, 1992). Phosphorylation of Ser392 in p53 is associated with formation of human tumors (Saito et al., 2003, Pise-Masison et al., 1998, Kim et al., 2004). In addition, p53 has been linked to effects of aging and oxidative stress (Sharpless and DePinho, 2002). An increase in p53 has also been linked to deficits in LTP and learning and memory (Jiang et al., 1998). Anti-Phospho Ser392 p53 Western blot of rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k p53 phosphorylated at Ser392 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho Ser392 p53 antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Molekulargewicht: '53 kDa

Gen-ID: 7157

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt