PI4K2A Antikörper (N-Term)

Produktnummer ABIN360472

Produktdetails anti-PI4K2A Antikörper

Target: PI4K2A (Phosphatidylinositol 4-Kinase Type 2 alpha (PI4K2A))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PI4K2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody reacts to PI4K2A.

Aufreinigung: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PI4K II.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu PI4K2A

Target: PI4K2A (Phosphatidylinositol 4-Kinase Type 2 alpha (PI4K2A))

Andere Bezeichnung: PI4K2A (PI4K2A Produkte)

Synonyme: pi4k2 antikoerper, pi4kii antikoerper, PI4KII antikoerper, PIK42A antikoerper, RP11-548K23.6 antikoerper, Pi4k2 antikoerper, Pi4KII antikoerper, pi4kII alpha antikoerper, wu:fc84f05 antikoerper, zgc:63646 antikoerper, phosphatidylinositol 4-kinase type 2 alpha antikoerper, phosphatidylinositol 4-kinase type 2 alpha L homeolog antikoerper, PI4K2A antikoerper, pi4k2a antikoerper, pi4k2a.L antikoerper, Pi4k2a antikoerper

Hintergrund: Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs. PI4K II is activated by detergent and inhibited by adenosine. Overexpression of PI4KII in COS-7 cells increases synthesis of PtdIns4P. Some cells overexpressing PI4KII have scattered or no perinuclear Golgi. Knockdown of PI4KII by RNA interference (RNAi) does not disrupt the Golgi, and some cells show expanded Golgi. RNAi reduces the Golgi level of PtdIns4P and blocks the association between AP1 and the trans-Golgi network. PI4KII RNAi had little effect on intra-Golgi trafficking, but it inhibited export to plasma membrane export by 35 % . It has been proposed that PI4KII generates PtdIns4P-rich domains within the Golgi that specify docking of the AP1 coat machinery.Synonyms: Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha

Gen-ID: 55361, 9606

UniProt: Q9BTU6

Pathways: Inositol Metabolic Process, Synaptic Membrane