V5 Epitope Tag Antikörper
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- Target Alle V5 Epitope Tag Produkte
- V5 Epitope Tag
- Reaktivität
- Bitte anfragen
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser V5 Epitope Tag Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Sequenz
- GKPIPNPLLG LDST
- Aufreinigung
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Immunogen
- KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
- Isotyp
- Ig Fraction
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- Applikationshinweise
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ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.25 mg/mL
- Buffer
- PBS containing 0.09 % (w/v) Sodium Azide as preservative.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- V5 Epitope Tag
- Andere Bezeichnung
- Pk (V5) Epitope Tag (GKPIPNPLLGLDST) (V5 Epitope Tag Produkte)
- Substanzklasse
- Tag
- Hintergrund
- Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). This polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
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