PGBD1 Antikörper (AA 230-241)

Produktnummer ABIN342993

Produktdetails anti-PGBD1 Antikörper

Target: PGBD1 (PiggyBac Transposable Element Derived 1 (PGBD1))

Bindungsspezifität: AA 230-241

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PGBD1 Antikörper ist unkonjugiert

Applikation: ELISA

Spezifität: Human PGBD1.

Homologie: Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Gibbon (92%), Orangutan (83%), Monkey (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-EKWSHLSLTRRN from an internal region of human PGBD1 (NP_115896.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Gibbon (92%), Orangutan (83%), Monkey (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: Peptide-ELISA (1:4000)

Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments gave bands at approx 150kD and 17kD in lysates of cell lines HeLa, HepG2 and K562 after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 92.5kD according to NP_115896.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Not recommended for: IHC-P

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PGBD1

Target: PGBD1 (PiggyBac Transposable Element Derived 1 (PGBD1))

Andere Bezeichnung: PGBD1 (PGBD1 Produkte)

Synonyme: 4921509E05Rik antikoerper, PGBD1 antikoerper, DKFZp469J1439 antikoerper, HUCEP-4 antikoerper, SCAND4 antikoerper, dJ874C20.4 antikoerper, piggyBac transposable element derived 1 antikoerper, PGBD1 antikoerper, Pgbd1 antikoerper

Hintergrund: Name/Gene ID: PGBD1

Synonyms: PGBD1, Cerebral protein 4, Cerebral protein-4, DJ874C20.4, HUCEP-4, SCAND4

Gen-ID: 84547

NCBI Accession: NP_115896

UniProt: Q96JS3