SEMA4D
Reaktivität: Human
WB, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 1 mL distilled water
Konzentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 5 % sucrose, 0.09 % Sodium Azide as preservative, 5 % Sucrose and 1 % BSA as stabilizer.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
This product is photosensitive and should be protected from light
Lagerung
4 °C
Informationen zur Lagerung
Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
Target
SEMA4D/CD100 (SEMA4D)
(Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))
Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.Synonyms: A8, BB18, GR3, SEMAJ, Semaphorin-4D