ARFIP1 Antikörper

Produktnummer ABIN317818

Produktdetails anti-ARFIP1 Antikörper

Target: ARFIP1 (ADP-Ribosylation Factor Interacting Protein 1 (ARFIP1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARFIP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of Arfaptin-1 protein.(region surrounding Lys314)

Aufreinigung: Affinity Chromatography using epitope-specific immunogen

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ARFIP1

Target: ARFIP1 (ADP-Ribosylation Factor Interacting Protein 1 (ARFIP1))

Andere Bezeichnung: Arfaptin-1 (ARFIP1 Produkte)

Synonyme: arfip1 antikoerper, MGC53116 antikoerper, DKFZp469C2322 antikoerper, HSU52521 antikoerper, 2310030I10Rik antikoerper, AW123087 antikoerper, Arfaptin antikoerper, D3Ertd598e antikoerper, ADP ribosylation factor interacting protein 1 antikoerper, ADP ribosylation factor interacting protein 1 L homeolog antikoerper, Arfaptin-1 antikoerper, ADP-ribosylation factor interacting protein 1 antikoerper, ARFIP1 antikoerper, arfip1.L antikoerper, arfp1 antikoerper, Arfip1 antikoerper

Hintergrund: Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.Synonyms: ADP-ribosylation factor-interacting protein 1, ARFIP1

Molekulargewicht: approx. 40 kDa

Gen-ID: 27236

NCBI Accession: NP_001020764

UniProt: P53367