ELISA: 1/5000-1/10000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Syndecans are type I integral membrane proteoglycans that contain both chondroitin sulfate and heparan sulfate groups. Syndecans are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 (SYND1, also called CD138) is an 85-92 kDa extracellular matrix receptor, which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 (also designated N-Syndecan) interact with MK (midkine), a growth/differentiation factor invloved in embryogenesis of the central nervous system. Syndecan-2 (also designated Fibroglycan) is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 (also designated Amphiglycan or Ryudocan) functions cooperatively with integrins in the processes of cell spreading, focal adhesion assembly and actin stress fiber assembly.Synonyms: Amphiglycan, Ryudocan core protein, SYND4