PRKCSH Antikörper

Produktnummer ABIN317664

Produktdetails anti-PRKCSH Antikörper

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKCSH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Spezifität: This antibody detects endogenous levels of Glucosidase II beta protein.(region surrounding Lys117)

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PRKCSH

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Andere Bezeichnung: Glucosidase 2 Subunit beta (PRKCSH Produkte)

Synonyme: AGE-R2 antikoerper, G19P1 antikoerper, PCLD antikoerper, PKCSH antikoerper, PLD1 antikoerper, 80K-H antikoerper, VASAP-60 antikoerper, zgc:55653 antikoerper, prkcsh antikoerper, MGC75615 antikoerper, MGC82696 antikoerper, PRKCSH antikoerper, protein kinase C substrate 80K-H antikoerper, protein kinase C substrate 80K-H L homeolog antikoerper, phospholipase D1 antikoerper, PRKCSH antikoerper, Prkcsh antikoerper, prkcsh antikoerper, prkcsh.L antikoerper, Bm1_42055 antikoerper, LOC100304710 antikoerper, LOC100526729 antikoerper, PLD1 antikoerper

Hintergrund: Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains alpha and beta subunits (Glucosidase IIalpha and Glucosidase IIbeta). The alpha and beta subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase IIalpha is the catalyitc core of the enzyme and can function independently of the beta subunit. The sequence of Glucosidase IIbeta encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase IIbeta is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIbeta was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIbeta retains the complete complex in the ER.Synonyms: 80K-H protein, G19P1, Glucosidase II beta, PRKCSH, Protein kinase C substrate 60.1 kDa protein heavy chain

Molekulargewicht: approx. 59 kDa

Gen-ID: 5589

NCBI Accession: NP_001001329

UniProt: P14314

Pathways: Cellular Glucan Metabolic Process, Methionine Biosynthetic Process