ELAVL1 Antikörper (AA 1-13)

Produktnummer ABIN307186

Produktdetails anti-ELAVL1 Antikörper

Target: ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))

Bindungsspezifität: AA 1-13

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Huhn, Hund, Schaf, Affe, Fledermaus, Kaninchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ELAVL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Marke: IHC-plus™

Spezifität: Recognizes human HuR, Mr ~36kD.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Horse, Pig, Opossum, Chicken (100%) Gibbon, Platypus (92%).

Aufreinigung: Affinity purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to aa1-13 (MSNGYEDHMAEDC) of human HuR. The immunizing sequence is identical in chicken, mouse, and rat. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Horse, Pig, Opossum, Chicken (100%), Gibbon, Platypus (92%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2.0 μg/mL. Detected HuR in RIPA lysates from 3T3/A31 cells. Immunoprecipitation: 4 μg immunoprecipitated HuR from 500 μg of 3T3/A31 RIPA lysate.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as a liquid in Tris-glycine, 0.15M sodium chloride, 0.05 % sodium azide,  pH 7.4, 30 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ELAVL1

Target: ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))

Andere Bezeichnung: ELAVL1 / HUR (ELAVL1 Produkte)

Synonyme: ELAV1 antikoerper, HUR antikoerper, Hua antikoerper, MelG antikoerper, HuR antikoerper, 2410055N02Rik antikoerper, W91709 antikoerper, elra antikoerper, fa25f08 antikoerper, fb59a02 antikoerper, fc60g02 antikoerper, hua antikoerper, wu:fa25f08 antikoerper, wu:fb59a02 antikoerper, wu:fc60g02 antikoerper, zHuA antikoerper, Elavl1 antikoerper, Xel-1 antikoerper, elav1 antikoerper, elrA antikoerper, hur antikoerper, melg antikoerper, p36 antikoerper, ElrA antikoerper, elavl1 antikoerper, elavl1-a antikoerper, elavl1-b antikoerper, ELAV like RNA binding protein 1 antikoerper, ELAV-like protein 1 antikoerper, ELAV (embryonic lethal, abnormal vision)-like 1 (Hu antigen R) antikoerper, ELAV like RNA binding protein 1a antikoerper, ELAV like RNA binding protein 1 L homeolog antikoerper, ELAV like RNA binding protein 1 S homeolog antikoerper, ELAVL1 antikoerper, Elavl1 antikoerper, elav1 antikoerper, elavl1a antikoerper, elavl1.L antikoerper, elavl1.S antikoerper

Hintergrund: Name/Gene ID: ELAVL1

Synonyms: ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG

Gen-ID: 1994

UniProt: Q15717

Pathways: AMPK Signaling